grade
Molecular Biology
Quality Level
form
powder blend
technique(s)
Southern blotting: suitable
pH
6.8-7.2(reconstituted per directions)
solubility
H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)
storage temp.
room temp
General description
酪蛋白是一种牛奶来源的物质，通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。
Application
适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后，封闭膜可用于化学发光，比色或 DIG 检测分析。
Features and Benefits
- 封闭性能优于奶粉或 BSA
- 与中性和正电荷膜兼容
- 与化学发光，比色和 DIG 检测分析兼容
Preparation Note
将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合，然后加水至终体积 1L。在 2-8°C 下最多保存一周。
存储类别
11 - Combustible Solids
wgk
WGK 1
法规信息
监管及禁止进口产品
此项目有
实验方案
Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
相关内容
Instructions
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
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Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
W Querbes et al.
Journal of virology, 80(19), 9402-9413 (2006-09-16)
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C7594-1L | 04061833513040 |