产品名称
组蛋白去乙酰化酶 3 (HDAC3) 活性检测试剂盒, 100 assays in 96 well plates
usage
100 assays in 96 well plates
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... HDAC3(8841)
mouse ... HDAC3(15183)
Features and Benefits
- 简单灵敏可靠的测定
- 程序简单；需要约60分钟
- 使用荧光测定法
- 样品类型：细胞和组织裂解物、血浆和血清、其他生物体液
- 种属反应性：哺乳动物
- 适用于独立测试或高通量分析和动力学研究
- 方便的96孔微孔板规格
- 适用于纯化，免疫沉淀和重组或基因修饰的HDAC3样品中HDAC3活性的高通量测量
General description
组蛋白脱乙酰化酶（HDAC）是从组蛋白中去除乙酰基的一大类酶。 已显示位点特异性组蛋白乙酰化和脱乙酰化分别激活或抑制真核基因转录，因此，它在哺乳动物发育和疾病中起关键作用。HDACs参与重要的生物学活动，例如细胞分化，增殖，凋亡和衰老。
使用Sigma′s HDAC3活性测定试剂盒，试样中存在的HDAC3将与提供的显影剂一起作用，使其脱乙酰化，然后裂解HDAC3底物[R-H-K-K（Ac）-AFC]。 该活性将释放淬灭的荧光基团AFC，可以在Em/Ex=380/500 nm处检测到。 曲古抑菌素A是试剂盒中包含的HDAC抑制剂，用于验证HDAC3活性。该试剂盒提供了快速，简单，灵敏和可靠的测试。 它适用于来自核提取物，纯化或免疫沉淀的HDAC3以及天然，重组或基因修饰的HDAC3的单独测试或高通量测定。
使用Sigma′s HDAC3活性测定试剂盒，试样中存在的HDAC3将与提供的显影剂一起作用，使其脱乙酰化，然后裂解HDAC3底物[R-H-K-K（Ac）-AFC]。 该活性将释放淬灭的荧光基团AFC，可以在Em/Ex=380/500 nm处检测到。 曲古抑菌素A是试剂盒中包含的HDAC抑制剂，用于验证HDAC3活性。该试剂盒提供了快速，简单，灵敏和可靠的测试。 它适用于来自核提取物，纯化或免疫沉淀的HDAC3以及天然，重组或基因修饰的HDAC3的单独测试或高通量测定。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
法规信息
监管及禁止进口产品
此项目有
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