跳转至内容
Merck
CN

F2637

Ficoll® 400

BioXtra, lyophilized powder

别名:

多聚蔗糖 400

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

化学文摘社编号:
UNSPSC Code:
12352200
NACRES:
NA.52
MDL number:
eCl@ss:
32190102
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助


biological source

non-animal source

Quality Level

grade

Molecular Biology

product line

BioXtra

form

lyophilized powder

foreign activity

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

storage temp.

room temp

General description

Ficoll® 400是蔗糖的非离子合成聚合物,可用于细胞分离和器官分离。

Application

用于细胞分离和细胞器分离。
适用于
  • 杂交实验,以减少标记探针与膜的非特异性结合
  • 制备DNA上样缓冲液(15% Ficoll®,0.25%溴酚蓝和0.25%二甲苯蓝FF)
  • 分离密度相似但大小不同的细胞(来自人外周血、骨髓和脐带血的PBMC)
  • 产生密度梯度,以便分离亚细胞成分
  • 细胞和组织样品的玻璃化。
  • 比较不同灌注液对缺氧性肺血管收缩(HPV)的影响。

Features and Benefits

  • 不含DNA酶、RNA酶、磷酸酶和蛋白酶
  • 可保留分离细胞的功能和形态完整性

Other Notes

一种非离子型合成蔗糖聚合物。

Legal Information

Ficoll is a registered trademark of Cytiva


Still not finding the right product?

Explore all of our products under Ficoll® 400


存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库


实验方案

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基,Ficoll 400®具有很强的亲水性和水溶性。

查看所有协议

商品

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.


Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Cristina Batlle et al.
Cell reports, 30(4), 1117-1128 (2020-01-30)
Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been



全球贸易项目编号

货号GTIN
F2637-25G04061835516032
F2637-100G04061835572977
F2637-10G04061835572984
F2637-5G04061835572991