227-037-7

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Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Assay of Betamethasone Sodium Phosphate by HPTLC

Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.

Thermal Transitions of Homopolymers: Glass Transition & Melting Point

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

CRISPR / Cas核酸酶RNA介导的基因组编辑

了解CRISPR Cas9、定义和工作原理。CRISPR是一种全新、实惠的基因组编辑工具，可让所有人掌握基因组编辑。

裂解酶

裂解酶提供了一种非机械式细胞裂解和原生质体准备方法。这看上去是个非常简单的过程：只需加入酶，将试管放入水浴然即可。但这过程中到底发生了什么？

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