Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.

Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase.

Antimicrobial agents and chemotherapy (2007-09-19)

Fátima Fonseca, Ana Cristina Sarmento, Isabel Henriques, Bart Samyn, Jozef van Beeumen, Pedro Domingues, Maria Rosário Domingues, Maria José Saavedra, António Correia

PMID17875998

摘要

The carbapenem-hydrolyzing beta-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.

材料

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品牌

产品描述

E9884

Sigma-Aldrich

乙二胺四乙酸, ACS reagent, 99.4-100.6%, powder

E6758

Sigma-Aldrich

乙二胺四乙酸, anhydrous, crystalline, BioReagent, suitable for cell culture

E8008

Sigma-Aldrich