Gliding Assay to Analyze Microtubule-based Motor Protein Dynamics.

Gliding Assay to Analyze Microtubule-based Motor Protein Dynamics.

Bio-protocol (2017-04-05)

Albert Shim, Gin Tezuka, Luke Kupcha, Li Tao

摘要

The purpose of this protocol is to provide an updated method of performing microtubule gliding assays and visualizing it using fluorescence microscopy.

材料

货号

品牌

产品描述

T1503

Sigma-Aldrich

Trizma ^® 碱, Primary Standard and Buffer, ≥99.9% (titration), crystalline

D9779

Sigma-Aldrich

DL-二硫苏糖醇, for molecular biology, ≥98% (HPLC), ≥99% (titration)

G8877

Sigma-Aldrich

鸟苷5′-三磷酸钠盐水合物, ≥95% (HPLC), powder

P7626

Sigma-Aldrich

苯甲磺酰氟, ≥98.5% (GC)

D9434

Sigma-Aldrich

葡萄糖, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications

G7141

Sigma-Aldrich

葡萄糖氧化酶来源于黑曲霉, Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)

P5405

Sigma-Aldrich

氯化钾, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%

E3889

Sigma-Aldrich

乙二醇-双（2-氨基乙醚）-N,N,N′,N′-四乙酸, for molecular biology, ≥97.0%

E3876

Sigma-Aldrich

N -乙基马来酰亚胺, crystalline, ≥98% (HPLC)

T6522

Sigma-Aldrich

胰蛋白酶抑制剂来源于大豆, powder, BioReagent, suitable for cell culture

L2884

Sigma-Aldrich

亮肽素, microbial, ≥90% (HPLC)

A6419

Sigma-Aldrich

腺苷 5'-三磷酸二钠盐水合物, microbial, BioReagent, suitable for cell culture, ≥99% (HPLC)

P6757

Sigma-Aldrich

哌嗪-N,N'-二(2-乙磺酸), ≥99% (titration)

P5368

Sigma-Aldrich

Phosphate buffered saline, BioPerformance Certified, pH 7.4

C7078

Sigma-Aldrich

酪蛋白来源于牛奶, technical grade

C1345

Sigma-Aldrich

过氧化氢酶来源于牛肝脏, powder, suitable for cell culture, 2,000-5,000 units/mg protein

P5318

Sigma-Aldrich

胃酶抑素 A, microbial, ≥90% (HPLC)

A3428

Sigma-Aldrich

抑肽酶来源于牛肺, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture

M4880

Sigma-Aldrich

氯化镁, BioReagent, suitable for insect cell culture, ≥97.0%