- Circadian cycle-dependent MeCP2 and brain chromatin changes.
Circadian cycle-dependent MeCP2 and brain chromatin changes.
PloS one (2015-04-16)
Alexia Martínez de Paz, Jose Vicente Sanchez-Mut, Mireia Samitier-Martí, Paolo Petazzi, Mauricio Sáez, Karolina Szczesna, Dori Huertas, Manel Esteller, Juan Ausió
PMID25875630
摘要
Methyl CpG binding protein 2 (MeCP2) is a chromosomal protein of the brain, very abundant especially in neurons, where it plays an important role in the regulation of gene expression. Hence it has the potential to be affected by the mammalian circadian cycle. We performed expression analyses of mice brain frontal cortices obtained at different time points and we found that the levels of MeCP2 are altered circadianly, affecting overall organization of brain chromatin and resulting in a circadian-dependent regulation of well-stablished MeCP2 target genes. Furthermore, this data suggests that alterations of MeCP2 can be responsible for the sleeping disorders arising from pathological stages, such as in autism and Rett syndrome.
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酒精, BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)
Supelco
十二烷基硫酸钠, dust-free pellets, suitable for electrophoresis, Molecular Biology, ≥99.0% (GC)
Sigma-Aldrich
酒精, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
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十二烷基硫酸钠, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate