跳转至内容
Merck
CN
  • Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode.

Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode.

Marine biotechnology (New York, N.Y.) (2015-08-06)
Sumihiro Koyama, Shinro Nishi, Maki Tokuda, Moeka Uemura, Yoichi Ishikawa, Takeshi Seya, Seinen Chow, Yuji Ise, Yuji Hatada, Yoshihiro Fujiwara, Taishi Tsubouchi
摘要

The purpose of this study was to develop a novel electrical retrieval method (ER method) for living sponge-associated microorganisms from marine sponges frozen at -80 °C. A -0.3-V vs. Ag/AgCl constant potential applied for 2 h at 9 °C induced the attachment of the sponge-associated microorganisms to an indium tin oxide/glass (ITO) or a gallium-doped zinc oxide/glass (GZO) working electrode. The electrically attached microorganisms from homogenized Spirastrella insignis tissues had intact cell membranes and showed intracellular dehydrogenase activity. Dead microorganisms were not attracted to the electrode when the homogenized tissues were autoclaved for 15 min at 121 °C before use. The electrically attached microorganisms included cultivable microorganisms retrieved after detachment from the electrode by application of a 9-MHz sine-wave potential. Using the ER method, we obtained 32 phyla and 72 classes of bacteria and 3 archaea of Crenarchaeota thermoprotei, Marine Group I, and Thaumarchaeota incertae sedis from marine sponges S. insignis and Callyspongia confoederata. Employment of the ER method for extraction and purification of the living microorganisms holds potential of single-cell cultivation for genome, transcriptome, proteome, and metabolome analyses of bioactive compounds producing sponge-associated microorganisms.

材料
Product Number
品牌
产品描述

Sigma-Aldrich
D -(+)-葡萄糖, ≥99.5% (GC)
Sigma-Aldrich
D -(+)-葡萄糖, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
纯乙醇, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
氯化钠, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
氯化钠 溶液, 5 M in H2O, BioReagent, Molecular Biology, suitable for cell culture
Sigma-Aldrich
氯化钠, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
氯化钠, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
氯化钠 溶液, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
葡萄糖, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications
Sigma-Aldrich
纯乙醇, 190 proof, ACS spectrophotometric grade, 95.0%
Sigma-Aldrich
D -(+)-半乳糖, ≥99% (HPLC), BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
D -(+)-葡萄糖, ≥99.5% (GC), BioXtra
Sigma-Aldrich
氯化钠 溶液, 5 M
SAFC
氯化钠 溶液, 5 M
Sigma-Aldrich
蔗糖, ACS reagent
Sigma-Aldrich
D -(+)-半乳糖, ≥99% (HPLC)
Sigma-Aldrich
D -(+)-葡萄糖, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
Sigma-Aldrich
硫酸镁, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
D -(+)-葡萄糖, ACS reagent
Sigma-Aldrich
纯乙醇, 190 proof, meets USP testing specifications
Sigma-Aldrich
氯化钠, BioUltra, Molecular Biology, ≥99.5% (AT)
Sigma-Aldrich
氯化钠, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sigma-Aldrich
氯化钠 溶液, BioUltra, Molecular Biology, ~5 M in H2O
Sigma-Aldrich
氯化钠, 99.999% trace metals basis
Sigma-Aldrich
硫酸镁 溶液, Molecular Biology, 1.00 M±0.04 M
Sigma-Aldrich
D -(+)-半乳糖, ≥98% (HPLC)
Sigma-Aldrich
蔗糖, puriss., meets analytical specification of Ph. Eur., BP, NF
Sigma-Aldrich
硫酸镁 溶液, BioUltra, Molecular Biology
Sigma-Aldrich
D -(+)-葡萄糖, suitable for mouse embryo cell culture, ≥99.5% (GC)
Sigma-Aldrich
硫酸镁, ≥99.99% trace metals basis