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Conformation of carboxylated schizophyllan in aqueous solution.

Conformation of carboxylated schizophyllan in aqueous solution.

Carbohydrate polymers (2015-10-03)

Kazuto Yoshiba, Takahiro Sato, Takaaki Osumi, Ann-Sissel T Ulset, Bjørn E Christensen

摘要

Carboxylated schizophyllan (sclerox) samples of different degrees of oxidation were molecularly characterized by size exclusion chromatography equipped with a multi-angle light scattering detector (SEC-MALS) in 0.10 M aqueous NaCl solution. The molar mass distribution obtained by SEC-MALS shows that sclerox of low degree of oxidation is dissolved mainly as the trimer, whereas the trimer and single chain coexist in solution of sclerox of high degree of oxidation. The trimer of sclerox is much more flexible than the fully ordered triple helix of the parent schizophyllan and easily dissociates into single chains upon heating.

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