GC Analysis of a 38-Component FAME Mix on SP®-2560, 200 m Column

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CONDITIONS
column
SP-2560, 200 m x 0.25 mm I.D., 0.20 μm (29688-U)
oven
100 °C (13 min), 4 °C/min to 240 °C (30 min)
inj. temp.
250 °C
detector
FID, 250 °C
carrier gas
hydrogen, 27 cm/sec
injection
1.0 μL, 50:1 split
liner
4 mm I.D., split type, cup design
sample
Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME
说明
分析说明
Identification and quantitation of individual fatty acid isomers in complex mixtures of isomers is desirable for both food and clinical scientists. This application requires the fatty acids to be converted to fatty acid methyl esters (FAMEs), followed by analysis using a long, efficient GC column with a stationary phase highly selective for polarizable analytes (those with double and/or triple C-C bonds). A 200 m SP-2560 is such a column.
法律信息
SP is a registered trademark of Sigma-Aldrich Co. LLC