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GC Analysis of a 38-Component FAME Mix on SP®-2560, 200 m Column

GC Analysis of a 38-Component FAME Mix on SP®-2560, 200 m Column suitable for GC

材料

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SP®-2560毛细管GC色谱柱

L × I.D. 200 m × 0.25 mm, df 0.20 μm

CONDITIONS

column

SP-2560, 200 m x 0.25 mm I.D., 0.20 μm (29688-U)

oven

100 °C (13 min), 4 °C/min to 240 °C (30 min)

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

hydrogen, 27 cm/sec

injection

1.0 μL, 50:1 split

liner

4 mm I.D., split type, cup design

sample

Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME

说明

分析说明

Identification and quantitation of individual fatty acid isomers in complex mixtures of isomers is desirable for both food and clinical scientists. This application requires the fatty acids to be converted to fatty acid methyl esters (FAMEs), followed by analysis using a long, efficient GC column with a stationary phase highly selective for polarizable analytes (those with double and/or triple C-C bonds). A 200 m SP-2560 is such a column.

法律信息

SP is a registered trademark of Sigma-Aldrich Co. LLC