HPLC Analysis of 25-Dihydroxyvitamin D₂, 25-Hydroxyvitamin D₃ and 3-epi-25-Hydroxyvitamin D₃ on Ascentis^® Express F5, 30 °C

HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C application for HPLC

材料

used together

产品编号

说明

价格

70221

甲酸铵

eluent additive for LC-MS, LiChropur^™, ≥99.0%

705993

3-表-25-羟基维生素 D₃

98% (CP)

53569-U

Ascentis^® Express F5, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

H-073

25-羟基维生素D2标准液 CRM 溶液

50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®

H-083

25-羟基维生素D_{3_{标准液 CRM 溶液}}

100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] 7 mM ammonium formate; [B] methanol (25:75, A:B)

flow rate

0.4 mL/min

pressure

4293 psi (296 bar)

column temp.

30 °C

detector

UV, 265 nm

ESI(+), m/z 100 - 1000

injection

1 μL

sample

300 mg/L in water:methanol

说明

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Applications

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HPLC Analysis of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3 and 3-epi-25-Hydroxyvitamin D3 on Ascentis® Express F5, 30 °C

材料