HPLC Analysis of Analgesics on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 10 mM ammonium acetate, 0.1% formic acid; [B] methanol

flow rate

1.5 mL/min

column temp.

35 °C

detector

UV, 254nm

injection

10 μL