HPLC Analysis of Anti-Inflammatory Drugs (Aminopyridine and Piroxicam) on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 10 mM potassium phosphate (pH 2.5); [B] acetonitrile; (85:15, A:B)

flow rate

1 mL/min

detector

UV, 220 nm

injection

5 μL