Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
[A] water with 1% acetic acid; [B] methanol; (85:15, A:B)
1 mL/min
35 °C
UV, 254 nm
10 μL
50 μg/mL each in mobile phase
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany