HPLC Analysis of Anxiolytic Drugs on Ascentis^® Phenyl

column

Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)

mobile phase

[A] 10 mM ammonium acetate (pH 5.5 with acetic acid): [B] acetonitrile; (40:60, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

10 μL

sample

50 μg/mL in 90:10, water:methanol