HPLC Analysis of Anxiolytic Drugs on SUPELCOSIL^™ LC-8

column

SUPELCOSIL LC-8, 15 cm × 4.6 mm I.D., 5 μm particles (58220-U)

mobile phase

methanol:acetonitrile:0.005 M KH₂PO₄ and 0.1 M ammonium acetate buffer (pH 6.0 with glacial acetic acid),26.5:16.5:57 (v/v/v)

flow rate

2 mL/min

column temp.

30 °C

detector

UV, 245 nm

injection

10 μL