HPLC Analysis of Benzodiazepines on Ascentis® C8

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CONDITIONS
column
Ascentis C8, 15 cm x 2.1 mm I.D., 3 μm particles (581402-U)
mobile phase
[A] 10 mM ammonium acetate (pH 6.98 unadjusted); [B] acetonitrile; (55:45, A:B)
flow rate
0.2 mL/min
column temp.
35 °C
detector
MS, ESI (+), Selected Ion Recording Mode
injection
5 μL
sample
1 μg/mL each in 10 mM ammonium acetate (pH 6.98 unadjusted)
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany