HPLC Analysis of Chlorhexidine and Robenidine on Ascentis^® Phenyl

column

Ascentis Phenyl 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)

mobile phase

[A] water; [B] acetonitrile; [C] 500 mM ammonium formate, pH 3.0 with formic acid

gradient

10% C constant; 65% A, 25% B to 0% A, 90% B in 7 min; held for 2 min; to 65% A, 25% B in 0.5 min

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 273 nm

injection

10 μL

sample

100 ug/mL each in 70:30, 25 mM ammonium formate pH 3.0:acetonitrile