HPLC Analysis of Estrogens on Ascentis^® Express Biphenyl

材料

E1132

β-雌二醇

analytical standard

46542

α-雌二醇

VETRANAL^®, analytical standard

E-074

雌三醇溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®

E-075

雌酚酮溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®

64065-U

Ascentis^® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (64065-U)

mobile phase

[A] water; [B] acetonitrile, (60:40, A:B)

flow rate

0.4 mL/min

pressure

2900 psi (200 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

2 μL

sample

60 μg/mL each in 50:50, water:organic solvent

说明

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC Columns GC Columns & Accessories TLC Plates & Adsorbents Solid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLC Large Molecule HPLC Gas Chromatography (GC)TLC Solid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS

HPLC Analysis of Estrogens on Ascentis® Express Biphenyl

材料

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