HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Express C18
材料
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CONDITIONS
column
Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile; (85:15, A:B)
flow rate
1.2 mL/min
column temp.
35 °C
detector
UV, 254 nm
injection
4 μL
sample
200 ng/mL in 25:75, water: acetonitrile
说明
分析说明
This application demonstrates the suitability of the Ascentis Express C18 for the analysis of fluoroquinolones.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany