HPLC Analysis of Furosemide and Indapamide on Discovery^® C18

column

Discovery C18, 15 cm × 4.6 mm ID, 5 μm particles (504955)

mobile phase

[A] 10mM monobasic potassium phosphate, (pH 3.0 with H₃PO₄): [B] acetonitrile; (40:60, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 234 nm

injection

10 μL

sample

10 μg/mL each in mobile phase