HPLC Analysis of Fusarium Mycotoxins on Ascentis^® Express Phenyl-Hexyl

Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)

[A] water, acetonitrile, 90:10; [B] acetonitrile

0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min

0.3 mL/min

192 bar (2780 psi)

30 °C

UV, 218 nm

10 μL

5 mg/L ea. in 95:5, water:acetonitrile

The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany