HPLC Analysis of Fusarium Mycotoxins on Ascentis^® Express Phenyl-Hexyl

column

Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)

mobile phase

[A] water, acetonitrile, 90:10; [B] acetonitrile

gradient

0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min

flow rate

0.3 mL/min

pressure

192 bar (2780 psi)

column temp.

30 °C

detector

UV, 218 nm

injection

10 μL

sample

5 mg/L ea. in 95:5, water:acetonitrile