HPLC Analysis of Heterocyclic Amines (HCAs) on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] water (10 mM ammonium acetate, pH 4.0 with acetic acid); [B] methanol (v/v); (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

5 μg/mL

sample

50 μg/mL each in mobile phase