HPLC Analysis of Horny Goat Weed Extract Adulterated with Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl
材料
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说明
价格
Sildenafil solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Vardenafil dihydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
sample preparation
Solvent Desorption
extraction
Weigh approximately 100 mg of herbal supplement and add 10 mL of 50:50, water:ethanol
sample preparation
Sonicate 20 min
sample preparation
Filter with 0.22 μm PVDF (Z227498)
column
Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (53345-U)
mobile phase
[A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient
5% B held for 0.5 min; to 100% B in 9.5 min; 100% B held for 2 min
flow rate
0.4 mL/min
pressure
2030 psi (140 bar)
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
Extract 10mg/mL before filtration, adulteration 20μg/mL in 50:50, water:ethanol
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany