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HPLC Analysis of Mannose and Maltose Sugars on Ascentis® Express HILIC

HPLC Analysis of Mannose and Maltose Sugars on Ascentis® Express HILIC application for HPLC

材料

相关产品

产品编号
说明
价格

M8378

麦芽三糖

≥90% (HPLC)

M7753

麦芽七糖

≥60% (HPLC)

M9153

麦芽六糖

≥65% (HPLC)

53981-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

1375025

麦芽糖 一水合物

United States Pharmacopeia (USP) Reference Standard

1375182

甘露糖

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (25:75, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

说明

分析说明

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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