HPLC Analysis of Organic Acids on Chromolith^® HighResolution RP-18 endcapped

column

Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D. (1.52022) (1.52022.0001)

mobile phase

10 mM diammonium hydrogen phosphate, pH 2.7

flow rate

1.0 mL/min

pressure

464 psi 32 bar

column temp.

30 °C

detector

UV, 210 nm

injection

20 μL

sample

tartaric acid, malic acid, citric acid, each 1.0 mg/mL in water, succinic acid, 0.2 mg/mL in water