HPLC Analysis of Organic Acids on Discovery^® C18

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] methanol; [B] water, 0.1% TFA (40:60, A:B)

flow rate

1 mL/min

column temp.

20 °C

detector

UV, 254 nm

injection

10 μL

sample

1 mg/mL each analyte