HPLC Analysis of Procainamide and N-Acetylprocainamide on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 25 mM ammonium phosphate (pH 7): [B] acetonitrile; (65:35, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 280 nm

injection

5 μL

sample

50 μg/mL