HPLC Analysis of Propoxyphene and Methadone on Ascentis^® Phenyl

column

Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)

mobile phase

[A] water with 0.1% ammonium acetate: [B] methanol; (20:80, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL

sample

50 μg/mL in 20:80, water:methanol