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HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems

HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems application for HPLC

材料

used together

产品编号
说明
价格

Ammonium bicarbonate

BioUltra, ≥99.5% (T)

相关产品

产品编号
说明
价格

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

Uracil

≥99.0%

Thyroglobulin from bovine thyroid

(For Gel Filtration Chromatography)

IgG from rabbit serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steel

CONDITIONS

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)

mobile phase

[A] 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid; [B] 0.1 M ammonium bicarbonate (pH unadjusted)

flow rate

0.3 mL/min

column temp.

25 °C

detector

UV, 280 nm

detector

1000 psi

injection

2.5 μL

sample

in mobile phase

说明

分析说明

This application compares selectivity of proteins when chromotographed by size exclusion with different buffer systems.

法律信息

Zenix is a registered trademark of Sepax Technologies