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HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems

HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems application for HPLC

材料

used together

产品编号
说明
价格

碳酸氢铵

BioUltra, ≥99.5% (T)

相关产品

产品编号
说明
价格

肌红蛋白 来源于马心脏

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

尿嘧啶

≥99.0%

甲状腺球蛋白 来源于牛甲状腺

(For Gel Filtration Chromatography)

IgG 来源于兔血清

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

牛血清白蛋白 来源于牛血清

lyophilized powder, ≥96% (agarose gel electrophoresis)

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steel

CONDITIONS

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)

mobile phase

[A] 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid; [B] 0.1 M ammonium bicarbonate (pH unadjusted)

flow rate

0.3 mL/min

column temp.

25 °C

detector

UV, 280 nm

detector

1000 psi

injection

2.5 μL

sample

in mobile phase

说明

分析说明

This application compares selectivity of proteins when chromotographed by size exclusion with different buffer systems.

法律信息

Zenix is a registered trademark of Sepax Technologies