HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW
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TSKgel® BioAssist® Chelate HPLC Column
phase iminodiacetic acid, L × I.D. 5 cm × 7.8 mm, 10 μm particle size, PEEK hardwareTSKgel® Chelate-5PW HPLC Column
phase iminodiacetic acid, L × I.D. 5 cm × 5 mm, 10 μm particle size, glassCONDITIONS
column
TSKgel Chelate-5PW, 5 cm x 5 mm I.D., 10 μm particles (column saturated with Zn2+ ions) (814440)
mobile phase
[A] 20 mM HEPES, 0.5 M sodium chloride, 1 mM imidazole, pH 8 with sodium hydroxide; [B] 20 mM HEPES, 0.5 M sodium chloride, 20 mM imidazole, pH 8 with sodium hydroxide
gradient
30 min linear gradient from 1 mM to 20 mM imidazole in 20 mM HEPES-NaOH buffer, pH 8 containing 0.5 M NaCl
flow rate
0.8 mL/min
detector
UV, 280 nm
说明
其他说明
Data provided by Tosoh Bioscience LLC.
法律信息
TSKgel is a registered trademark of Tosoh Corporation