TSKgel Chelate-5PW, 5 cm x 5 mm I.D., 10 μm particles (column saturated with Zn2+ ions) (814440)
[A] 20 mM HEPES, 0.5 M sodium chloride, 1 mM imidazole, pH 8 with sodium hydroxide; [B] 20 mM HEPES, 0.5 M sodium chloride, 20 mM imidazole, pH 8 with sodium hydroxide
30 min linear gradient from 1 mM to 20 mM imidazole in 20 mM HEPES-NaOH buffer, pH 8 containing 0.5 M NaCl
0.8 mL/min
UV, 280 nm
Data provided by Tosoh Bioscience LLC.
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