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HPLC Analysis of Steroids on Ascentis® Express Biphenyl

HPLC Analysis of Steroids on Ascentis® Express Biphenyl application for HPLC

材料

分析色谱柱

产品编号
说明
价格

64059-U

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

标准

产品编号
说明
价格

E1132

β-雌二醇

analytical standard

D7000

醋酸去氧皮质酮

H3160

氢化可的松

meets USP testing specifications

C2800000

Cortisone acetate

European Pharmacopoeia (EP) Reference Standard

C-117

皮质酮标准液 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

E-074

雌三醇 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

P-069

孕酮 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

T-037

睾酮 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

C-130

可的松标准液 溶液

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express Biphenyl,  5 cm x 4.6 mm I.D., 2.7 μm particles (64059-U)

mobile phase

[A] water; [B] acetonitrile

gradient

20 to 60% B in 6 min

flow rate

1.85 mL/min

pressure

4998 psi (344 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

4 μL

sample

25 ng/mL each except 125 ng/mL deoxycorticosterone in 62.5:37.5, water:acetonitrile

说明

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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