HPLC Analysis of Tetracycline Antibiotics on Ascentis® Phenyl
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CONDITIONS
column
Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)
mobile phase
[A] 0.1% ammonium acetate; [B] acetonitrile with 0.1% ammonium acetate; (80:20, A:B)
flow rate
1.0 mL/min
column temp.
35 °C
detector
UV, 353 nm
injection
10 μL
sample
50 μg/mL each in 80:20, water:methanol
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany