SPE (Solid Phase Extraction)
rabbit plasma, unfiltered K2-EDTA, spiked with each compound at 100 ng/mL (3:1, plasma:1% formic acid in acetonitrile)
HybridSPE-Phospholipid, 96-well plate (575656-U)
to each well add 100 μL plasma, followed by a 300 μL of 1% formic acid in acetonitrile
collect, filtrate and analyze directly.
Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile, 5:95 (A:B), adjusted to pH 7.0 with formic acid
0.4 mL/min
1305 psi (90 bar)
35 °C
ESI+, 100-1000 m/z
0.5 μL
This application shows the extraction and analysis of antiarrhythmic cardiac drugs in plasma samples using LC/MS/MS. HILIC chromatography on Ascentis Express along with a selective sample preparation using HybridSPE-Phospholipid provided sensitivity and resolution. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany