LC/MS Analysis of Pilocarpine and Betaxolol in the Presence of Benzalkonium Chlorides on Ascentis® Express HILIC
CONDITIONS
column
Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase
[A] 2 mM arrmonium acetate, pH 6.7 in [B] 10:90 (v/v) water:acetonitrile
flow rate
1.0 mL/min
pressure
1160 psi (80 bar)
column temp.
35 °C
detector
ESI(+), XlC, m/z 304, 332 (BACs), m/z 209 (pilocarpine), m/z 308 (betaxolol)
injection
2 μL
sample
10 μg/mL each in acetonitrile
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany