LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
材料
分析色谱柱
标准
25-羟基维生素D2标准液 CRM 溶液
50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®25-羟基维生素D3标准液 CRM 溶液
100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®1α,25-二羟基维生素D3标准液 CRM 溶液
5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®1α,25-二羟基维生素D2标准液 溶液
5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express F5, 15 cm x 2.1 mm I.D., 2.7 μm particles (53571-U)
mobile phase
[A] 5 mM ammonium formate water; [B] methanol; (25:75, A:B)
flow rate
0.4 mL/min
pressure
5178 psi (357 bar)
column temp.
40 °C
detector
ESI(+), 100-1000 m/z
injection
2 μL, each compound 300 ng/mL in 40:60 (water:methanol)
说明
分析说明
The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity.This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany