Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile; (5:95, A:B, pH 7.0 with formic acid)
0.4 mL/min
35 °C
ESI(+), full scan, m/z 200-800
0.5 μL
each compound, 300 ng/mL in 1% formic acid acetonitrile:water, 75:25
The basic nature of these compounds makes them targets for HILIC chromatographic separation. HILIC mobile phases consist of a high composition of acetonitrile, which facilitates the direct analysis of precipitated plasma samples without the need for additional sample solvent exchange. In most cases, the high organic mobile phase also facilitates increased analyte response with ESI(+) MS detection. Separation was performed on an Ascentis Express HILIC Fused-Core HPLC column. High purity solvents provided clean, robust operation. Cerilliant CRMs provided reliable quantification.
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