Scalability of TSKgel^® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl^® Phenyl-650M Resins

column

TSKgel Phenyl-5PW, 7.5 cm x 7.5 mm, 20 μm particles (843277)

column

TSKgel Phenyl-5PW, 7.5 cm x 7.5 mm, 30 μm particles (843177)

column

TOYOPEARL Phenyl-650M, 7.5 cm x 7.5 mm, 65 μm particles (819818)

gradient

for the 10 and 30 μm TSKgel columns: 0 to 100% B in 60 min;

gradient

for the 65 μm TOYOPEARL columns: 0 to 100% B in 240 min

flow rate

for the 10 and 30 μm TSKgel columns: 68 cm/hr;

flow rate

for the 10 and 30 μm TSKgel columns: 68 cm/hr;

for the 65 μm TOYOPEARL columns: 272 cm/hr

detector

UV, 280 nm

injection

100 μL containing 1 mg/mL each protein