Typical Desalting Chromatograms on TSKgel® BioAssist® DS HPLC Columns, Increasing Sample Injection Volume
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TSKgel® BioAssist® Desalting HPLC Column
phase polyacrylamide, L × I.D. 15 cm × 10 mm, 13 μm particle size, PEEKTSKgel® BioAssist® Desalting HPLC Column
phase polyacrylamide, L × I.D. 15 cm × 4.6 mm, 15 μm particle size, PEEKCONDITIONS
column
TSKgel BioAssist DS, 15 cm x 10 mm I.D., 13 μm particles (821464)
mobile phase
50 mM sodium phosphate buffer, pH 6.5
flow rate
3 mL/min 50 mM sodium phosphate buffer, pH 6.5
column temp.
4 °C
detector
UV, 280 nm and refractive index (RI)
injection
10 μL
sample
10 mg/mL ovalbumin in 0.5 M NaCl and 25 mM sodium phosphate buffer, pH 6.5
说明
其他说明
Data provided by Tosoh Bioscience LLC.
法律信息
BioAssist is a registered trademark of Tosoh Corporation, TSKgel is a registered trademark of Tosoh Corporation