UHPLC Analysis of p-Acetophenetide (Phenacetin) Titan^™ C18

column

TITAN C18, 10 cm x 2. 1 mm I.D., 1.9 um particles (577124-U)

mobile phase

(A) 10 mM ammonium acetate, pH 7.0; (B) acetonitrile (70:30, A;B)

flow rate

0.3 mL/min

column temp.

35 °C

detector

MS, XIC m/z 180.10

injection

2 μL

sample

300 ng/mL each in acetonitrile:water (3:1)