Ascentis C18, 25 cm x 4.6 mm I.D., 5 μm particles (581325-U)
[A] water; [B] acetonitrile
10 to 50% B in 13 min; to 60% B in 10 min; to 90% B in 6 min; held for 5 min
1.0 mL/min
30 °C
UV, 210 nm
10 μL
as indicated in 16% acetonitrile in water
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany