US EPA Method 632: HPLC Analysis of Pesticides on Ascentis^® C8

column

Ascentis C8, 25 cm x 4.6 mm I.D., 5 μm particles (581425-U)

mobile phase

[A] DI water; [B] acetonitrile

gradient

15 to 25% B in 4 min; to 45% B in 40 min; to 60% B in 6 min; held for 15 min

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 210 nm

injection

10 μL

sample

as indicated in 16% acetonitrile in DI water