UHPLC Analysis of A Monoclonal Antibody System Suitability Standard on BIOshell IgG 1000 Å C4

column

BIOshell IgG 1000 Å C4; 10 cm x 2.1 mm I.D., 2.7 μm particles (63288-U)

mobile phase

[A] 70:30 0.1% TFA (water): 0.1% TFA (acetonitrile); [B] 50:50 0.1% TFA (water): 0.1% TFA (acetonitrile)

gradient

0% B to 50% B in 50 min; 50% B to 100% B in 10 min

flow rate

0.2 mL/min

pressure

1325 psi (91.4 bar)

column temp.

75 °C

detector

UV, 215 nm

injection

1 μL

sample

MMMonoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water)Monoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water) A03
4
A034Monoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water) A034MMMonoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water)Monoclonal Antibody System Suitability Standard, 1 g/L, 0.05% TFA (water)
A03