跳转至内容
Merck
CN

UHPLC Analysis of Catecholamines on Ascentis® Express F5

UHPLC Analysis of Catecholamines on Ascentis® Express F5 application for UHPLC

材料

used together

产品编号
说明
价格

乙酸铵

LiChropur, eluent additive for LC-MS

相关产品

产品编号
说明
价格

Ascentis® Express C18, 2.7 μm HPLC 色谱柱

L × I.D. 10 cm × 2.1 mm, pk of 1 ea

儿茶酚胺混合物1(肾上腺素) 溶液

1.0 mg/mL each component in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

儿茶酚胺混合物2(变肾上腺素) 溶液

1.0 mg/mL each component in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

多巴胺盐酸盐标准液 CRM 盐酸盐 溶液

1.0 mg/mL in methanol with 5% 1 M HCl (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] water; [B] 100 mM ammonium acetate (pH 4.1 with acetic acid); [C] acetonitrile; (88:2:10, A:B:C)

flow rate

0.3 mL/min

pressure

1880 psi (130 bar)

column temp.

60 °C

detector

UV, 250 nm

injection

Catecholamines 25 μg/mL in 90:10, water:methanol

说明

分析说明

The analysis of catecholamines in a clinical setting is often for the diagnosis of pheochromocytoma (a type of adrenal tumor) in symptomatic people. The analysis shown here on Ascentis Express F5 provides rapid resolution of five common catecholamines.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany