UHPLC Analysis of Quinidine and Metabolite on Titan C18

column

Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)

mobile phase

[A] 10 mM potassium phosphate, monobasic pH 3.0; [B] acetonitrile; (80:20, A:B)

flow rate

0.6 mL/min

pressure

4310 psi (297 bar)

column temp.

35 °C

detector

UV, 200 nm

injection

1 μL

sample

Quinidine 5 μg/mL in 80:20, water:methanol