CN
UHPLC Analysis of Temsirolimus and Sirolimus on Titan™ C18
CONDITIONS
column
Titan C18 10 cm x 2.1 mm, 1.9 μm particles (577124-U)
mobile phase
[A] 0.1% formic acid in water, [B] methanol
gradient
75% B for 2 minutes, 75% B to 90% B from 2 to 12 minutes, 90% B from 12 to 14 minutes.
flow rate
0.3 mL/min
pressure
3300 psi (228 bar)
column temp.
55 °C
detector
UV, 276 nm
injection
2.0 μL
sample
100 μg/mL each in 20% methanol
说明
分析说明
The anticancer agents temsirolimus and sirolimus are shown here resolved, efficienttly and rapidly, on a Titan C18 UHPLC column. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
法律信息
Titan is a trademark of Sigma-Aldrich Co. LLC