UHPLC Analysis of Temsirolimus and Sirolimus on Titan C18

Titan C18 10 cm x 2.1 mm, 1.9 μm particles (577124-U)

[A] 0.1% formic acid in water, [B] methanol

75% B for 2 minutes, 75% B to 90% B from 2 to 12 minutes, 90% B from 12 to 14 minutes.

0.3 mL/min

3300 psi (228 bar)

55 °C

UV, 276 nm

2.0 μL

100 μg/mL each in 20% methanol

The anticancer agents temsirolimus and sirolimus are shown here resolved, efficienttly and rapidly, on a Titan C18 UHPLC column. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

null