UHPLC/MS Analysis of Vitamin K1 and Related Compound on Ascentis® Express C18
CONDITIONS
column
Ascentis Express C18 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase
[A] methanol; [B] 2-propanol; (90:10, A:B)
flow rate
0.4 mL/min
pressure
1870 psi (129 bar)
column temp.
35 °C
detector
MS-APCI+, full scan 425-525 m/z
injection
2 μL
sample
50 ng/mL 90:10, methanol:2-propanol
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany