等级
Molecular Biology
产品线
BioReagent
表单
lyophilized powder
杂质
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
适用性
suitable for nucleic acid hybridization
储存温度
2-8°C
一般描述
Denhardt′s溶液是一种高分子量聚合物的混合物，可作为封闭剂使用。它可用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。该粉末可用于制备50倍浓缩物，其可稀释至任何浓度，用于各种预杂交和杂交缓冲液。
应用
适用于阻断western、northern和Southern印迹膜中的非特异性结合
制备说明
将全部内容物溶解在5 mL分子生物级水中，以制备50倍浓缩液。在−20°C下可储存长达两年。
其他说明
每瓶Denhardt′s粉末含有50 mG牛血清白蛋白（BSA）、50 mG 聚蔗糖和50 mG聚乙烯吡咯烷酮（PVP）。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
此项目有
Flavien Pillet et al.
Nucleic acids research, 39(17), 7477-7486 (2011-06-10)
The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp. Each
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual (1989)
Ren Li et al.
Nature communications, 11(1), 5844-5844 (2020-11-19)
Fruit firmness is a target trait in tomato breeding because it facilitates transportation and storage. However, it is also a complex trait and uncovering the molecular genetic mechanisms controlling fruit firmness has proven challenging. Here, we report the map-based cloning
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