Enzyme activity assays

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facet applications:Enzyme activity assays

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转化酶的酶学测定

在测定转化酶活性时，使用了一种在410nm处的分光光度终点反应法进行测定。在55 ºC、pH 4.5条件下，一单位的转化酶每分钟可将1.0 μmole的蔗糖水解为转化糖。

6-磷酸葡萄糖脱氢酶（EC 1.1.1.49）的酶学测定

在测定6-磷酸葡萄糖脱氢酶活性时，使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

四唑盐法(MTT)细胞活力和增殖检测方案

检测细胞活力、生长增殖和毒性的四唑盐法(MTT)。四唑盐(MTT)试剂制备说明和应用示例。

改良Boehenger法酶促检测α-葡萄糖苷酶(EC 3.2.1.20)

淀粉葡萄糖苷酶的酶学测定（EC 3.2.1.3）

本操作程序可用于使用淀粉作为底物测定淀粉葡萄糖苷酶的活性。

5'-核苷酸酶的酶促检测

在测定5′-核苷酸酶活性时，本操作流程使用5′-单磷酸腺苷和一种显色试剂生成的标准曲线而对释放的磷的微摩尔数进行测定。

溶菌酶的酶学测定（EC 3.2.1.17）

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

植酸酶的酶促检测(EC 3.1.3.8)

源自操作步骤SSPHYT02。包括模板根据现行SOP规范更新和整合注意事项到操作步骤。

胰蛋白酶（EC 3.4.21.4）的酶学测定方案

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：

核糖核酸酶A(EC 3.1.27.5)的酶促测定

本实验方案可用于测定核糖核酸酶A(RNase A)的活性。

LPL Activity Assay Protocol

Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Assay Procedure for Xanthine Oxidase Microbial

The appearance of uric acid is measured at 293nm by spectrophotometry.

Enzymatic Method for Determining Phenylalanine (Phenylalanine Assay)

Assay protocol for the fluorometric detection of Phenylalanine in biological samples using the phenylalanine assay kit.

Assay Procedure for Glycerol Dehydrogenase

Enzymatic Assay of Neuraminidase

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

Assay Procedure for Lipase

Recycling Micro-Assay of β-NAD and β-NADH

Objective: To standardize a procedure for the recycling micro-assay of β-NAD and β-NADH

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

Enzymatic Assay of Trypsin Inhibitor

This technical article described the Enzymatic Assay of Trypsin Inhibitor.

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Alkaline Phosphatase (AP) Protocol

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

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