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Expanded multilocus sequence typing for burkholderia species.

Expanded multilocus sequence typing for burkholderia species.

Journal of clinical microbiology (2009-06-06)

Theodore Spilker, Adam Baldwin, Amy Bumford, Chris G Dowson, Eshwar Mahenthiralingam, John J LiPuma

摘要

PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

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